Catalytic metalloporphyrin protects against MPTP-induced Parkinson's disease in mice
10.3724/SP.J.1008.2008.00036
- Author:
Ping CHEN
1
Author Information
1. School of Life Science and Technology
- Publication Type:Journal Article
- Keywords:
MnTDM;
MPTP;
Oxidative stress;
Parkinson disease
- From:
Academic Journal of Second Military Medical University
2010;29(1):36-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of manganese (III) meso-tetrakis (N, N′-diethylimidazolium-2-yl) porphyrin (MnTDM) in treatment of early Parkinson's disease (PD) mouse model induced by subcutaneous injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP) and to discuss its possible mechanism. Methods: Forty male C57BL/6 mice were evenly randomized into 4 groups: MPTP model group (subcutaneous injection of 25 mg/kg MPTP for 3 days), MnTDM+MPTP group (15 mg/kg MnTDM was subcutaneously injected 1 h before MPTP injection), MnTDM control group, and normal saline group. Performance of animals in the pole and swimming test was observed 3 days after the last injection. Levels of dopamine (DA) and its metabolites (3,4-dihydroxyphenylacetic acid [DOPAC] and homovanillic acid [HVA] in the striatum of animals were measured by high-performance liquid chromatography with an electrochemical detector (HPLC-ECD). Thiobarbituric acid (TBA) method was used to examine the levels of malondialdehyde (MDA). Results: Acute injection of MPTP could be used for establishment of PD model. The striatal levels of DA, DOPAC and HVA in MPTP group were significantly lower (P<0.01) and the striatal level of MDA was significantly higher(P<0.05) than those of the control group. MPTP had no obvious effect on the behavioral performance of the animals in a short term. MnTDM could partly inhibit the above effects of MPTP. Compared with MPTP group, MnTDM+MPTP group had significantly higher DA, DOPAC, and HVA levels and significantly lower MDA level (all P<0.05). There was no significant difference in the behavioral indices of animals between the 4 groups. Conclusion: MnTDM can inhibit lipid peroxidation and promote DA production; it has preventive and therapeutic effects on MPTP-induced PD.
