Establishment and evaluation of a nicotine-treatment and -withdrawal rat model
10.3724/SP.J.1008.2008.00156
- Author:
Xiao-Hong LAN
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Animal models;
Aorta;
Bodymass;
Fats;
Nicotine;
Smoking;
Tobacco use cessation
- From:
Academic Journal of Second Military Medical University
2010;29(2):156-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a nicotine-treatment and -withdrawal rat model and to evaluate its characteristics and application through analyzing 3 parameters. Methods: Male Sprague-Dawley rats, aged 10-11 weeks old, were randomly divided into normal saline group(subcutaneous injection of saline [0.5 ml/kg] for 6 weeks), nicotine-treated group (injection with nicotine [0.5 ml/kg, 3 mg/(kg · d)] for 6 weeks), and nicotine-withdrawn group (injection with nicotine [0.5 ml/kg, 3 mg/(kg · d)] for 3 weeks and followed by saline injection for additional 3 weeks). Body weight, food intake, and water intake of animals were recorded during the treatment in 3 groups. The model was evaluated through analyzing body weight, serum parameters and adipose tissue weights. Results: The body weight of rats, as well as the serum levels of triglyceride and insulin, were all decreased after nicotine treatment; the weights of subcutaneous fat, visceral fat and periaortic fat were also decreased. The above indicators increased after withdrawal of nicotine. Conclusion: The established model can be used to study multiple pharmacological effects of nicotine; it can also be used for smoking and smoking cessation related studies.
