Expression of aquaporin 5 in pulmonary tissues of bronchial asthmatic mice and influence of dexamethasone on the expression
10.3724/SP.J.1008.2008.00126
- Author:
Chun-Ling DONG
1
Author Information
1. Department of Respiratory Medicine
- Publication Type:Journal Article
- Keywords:
Aquaporin 5;
Asthma;
Dexamethasone;
Mucins
- From:
Academic Journal of Second Military Medical University
2010;29(2):126-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of aquaporin 5 (AQP5) and MUC5AC in lung of bronchial asthmatic murine model and the regulatory effect of dexamethasone on the expression. Methods: Forty-eight female C57BL/6 mice were evenly randomized into control group, asthma group and dexamethasone group. The pathological changes of the lungs and the wet/dry weight ratios of the lungs were determined in mice of each group. The expression of AQP5 and MUC5AC mRNA in the lung was determined by real-time poly-merase chain reaction (real-time PCR); the AQP5 protein was examined by Western blotting assay; the distribution of AQP5 protein in the pulmonary tissue was studied immunohistochemically; and the content of MUC5AC in the bronchial alveolar lavage fluid (BALF) was determined by ELISA. Results: Compared with the control group, the pulmonary expression of AQP5 in asthma group was obviously decreased (mRNA by[42.5 ± 3.6]%, protein by [64.3 ± 8.2]%), while the expression of MUC5AC was increased (mRNA by[93.3±8.1]%,protein by[98.3%±7.2]%). Dexamethasone significantly decreased the pulmonary expression of AQP5 and MUC5AC compared with the asthma group (P<0.05). Conclusion: The decreased pulmonary expression of AQP5 in asthmatic mice might be related to the elevation of MUC5AC expression in the airway; dexamethasone can decrease AQP5 expression and therefore alleviate the overproduction of airway mucus, pulmonary inflammation and lung edema.
