Identification and antibacterial/antifungal activity of a strain of Bacillus from East China Sea
10.3724/SP.J.1008.2008.00321
- Author:
Xiao-Yu LIU
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- Keywords:
16S rDNA sequence analysis;
Anti bacterial activiy;
Baccillus;
Hapophilism
- From:
Academic Journal of Second Military Medical University
2010;29(3):321-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify a strain of bacterium (numbered ZJ8112) isolated from the sediments of the East China Sea and study its antibacterial activity. Methods: The ZJ8112 strain was subjected to phenotypical study, salt-aggregation test and 16S rDNA sequence analysis; the sequence analysis result was searched in the GenBank to determine its characteristics. The antibacterial activity of strain ZJ8112 was tested using the following as the indicator bacteria: Pyricularia oryzae, Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae, Candida albicans and Cladosporium cucumerinum. Results: We found that the ZJ8112 was a G+ bacterium and had central spore. Salt-tolerant study showed that the optimum salt concentration was 10% and it belonged to moderate halophile. Analysis of the 16S rDNA gene showed high similarity (99%) to the Bacillus subtilis F121112. The 16S rDNA sequence of strain ZJ8112 had been submitted to GenBank (accession number: EU100745). The cultural and phenotypical characteristics demonstrated that ZJ8112 is a strain of Bacillus. Bioactivity study showed that ZJ8112 had potent inhibitory activity on Escherichia coli and Pyricularia oryzae. Conclusion: ZJ8112 is a halophytic bacterium with an optimum salt concentration of 10%. ZJ8112 can strongly inhibit the Escherichia coli and Pyricularia oryzae,showing a potential role for antibiotics development.
