Effect of leptin on serum TNF-α and IL-10 levels and small intestine damage in rats with intestine ischemia/reperfusion injury
10.3724/SP.J.1008.2008.00250
- Author:
Xiao-Hui WANG
1
Author Information
1. Laboratory of Biochemistry
- Publication Type:Journal Article
- Keywords:
Cytokine;
Intestinal mucosa;
Leptin;
Reperfusion injury
- From:
Academic Journal of Second Military Medical University
2010;29(3):250-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the protective effect of leptin against rats intestinal ischemia/reperfusion injury and the related mechanism. Methods: Ninety-nine rats were randomly divided into 11 groups (n=9), namely the sham-operation group, ischemia/reperfusion groups (45 min ischemia plus 15 reperfusion [I45R15,I45R30,I45R60,I45R180,I45R360)and the 5 corresponding leptin-treated ischemia-reperfusion groups. The serum levels of TNF-α and IL-10 were determined in all groups and the histopathological changes were observed in the small intestine. Results: Compared with the sham-operation group, the serum levels of TNF-α and IL-10 were significantly increased in the ischemia/ reperfusion groups (both P<0.05), with obvious pathological damage observed in the ischemia/reperfusion groups. Leptin treatment significantly decreased TNF-α level, increased IL-10 level, and alleviated the damage of the small intestine (all P<0.05) of animals after ischemia/reperfusion injury. Conclusion: Leptin can alleviate the intestinal damage in rats after intestinal ischemia/reperfusion through down-regulating TNF-α level and up-regulating IL-10 levels.
