Role of p38MAPK in regulating expression of NF-κB and COX-2 in rat mesangial cell
10.3724/SP.J.1008.2008.00241
- Author:
Qian-Ping WEI
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase-2;
Diabetic nephropathy;
Mesangial cells;
Nuclear factor-kappa B;
p38 mitogen-activated protein kinases
- From:
Academic Journal of Second Military Medical University
2010;29(3):241-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the relationship between p38 mitogen-activated protein kinase (p38MAPK), nuclear factor-κB (NE-κB) and cyclooxygenase-2 (COX-2), so as to study the roles of p38MAPK, NF-κB nd COX-2 in diabetic nephropathy. Methods: Rat mesangial cell line HBZY-1 was incubated with certain concentrations of glucose(25 mmol/L), insulin (100 mmol/L), H2O2 (100 mmol/L) and glycation endproducts (AGEs) (100 mg/L) with or without pretreatment with SB203580, a specific inhibitor of p38MAPK, then the expression of p38MAPK, NF-κB and COX-2 in the HBZY-1 cells was examined. Results: High glucose, high insulin, H2O2 and AGEs independently activated p38MAPK, increased p38MAPK phosphorylation, and significantly up-regulated the expression of NF-κB and COX-2 compared with the control group (P<0.01). Pretreatment with SB203580 significantly decreased expression of NF-κB and COX-2 compared with the corresponding stimulating group (P<0.01). Conclusion: p38MAPK may induce renal damage during diabetic nephropathy by activating NF-κB and COX-2, indicating that p38MAPK, NF-κB and COX-2 may play important roles in the development of diabetic nephropathy.