GnRH analog resensitizes cisplatin-resistant human ovarian cancer cells
10.3724/SP.J.1008.2008.00395
- Author:
Dan WANG
1
Author Information
1. Department of Gynecology and Obstetrics
- Publication Type:Journal Article
- Keywords:
Cisplatin;
Drug tolerance;
Ovarian neoplasms;
Triptorelin
- From:
Academic Journal of Second Military Medical University
2010;29(4):395-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of GnRH analog triptorelin in resensitizing cisplatin-resistant human ovarian cancer cells and to discuss the related mechanism. Methods: Cisplatin-resistant human ovarian cancer cell line OVCAR-3/CDDP was established in vitro. MTT assay was used to assess the inhibitory effects of triptorelin, cisplatin alone or a combination of both on OVCAR-3/CDDP cells. Flow cytometry was employed to observe the expression changes in epidermal growth factor receptor (EGFR) in different groups. Results: The drug restant index of OVCAR-3/CDDP cells was 13.42. The resensitizing fold of cisplatin combined with triptorelin was 3.80. The expression of EGFR had the most prominent decrease in OVCAR-3/CDDP cells in the combination group. Conclusion: Triptorelin can partially resensitize cisplatin- resistant OVCAR-3/CDDP cells, which might be related to the down-regulation of EGFR.
