Effect of CART55-102 protein vaccine on morphine analgesia and tolerance
10.3724/SP.J.1008.2008.00349
- Author:
Juan SONG
1
Author Information
1. Department of Neurobiology
- Publication Type:Journal Article
- Keywords:
Analgesia;
Cocaine- and amphetamine-regulated transcript protein;
Drug tolerance;
Morphine;
Vaccine
- From:
Academic Journal of Second Military Medical University
2010;29(4):349-352
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of cocaine- and amphetamine-regulated transcript (CART) protein vaccine on morphine analgesia and tolerance. Methods: The expression plasmid pGEX-4T3-CART was constructed by gene cloning. The CART protein was purified by glutathione s-transferase (GST)-affinity chromatography. The experiment included 6 groups: blank control, normal saline (NS) GST + Freund's adjuvant, and CART protein vaccine (5 μg, 10 μg and 20 μg) groups. After immunization for twice, all groups were tested in hot plate. Morphine analgesia effect was evaluated through s. c. injection with 6 mg/kg morphine solution, calculated by MPE%. Then morphine tolerance model was established, and the tolerance to morphine was tested by s. c. injection with 6 mg/kg morphine 12 h after the last injection. Results: CART vaccine itself had no pronounced effect on the pain threshold (P>0.05). CART vaccine at 10 μg significantly depressed the analgesic effect of morphine analgesia (P<0.05). Compared with NS group, vaccine groups showed a potential antagonizing tolerance effect, especially in the 10 μg group, with the MPE% significantly increased (P<0. 05). Conclusion: CART vaccine itself has no influence on the pain response; however, it can impair the analgesia effect of morphine and can antagonize the analgesia tolerance to morphine.
