Preparation and culture of NIH3T3 cells harboring microencapsulated VEGF gene
10.3724/SP.J.1008.2008.00491
- Author:
Yan-Fu HAN
1
Author Information
1. Department of Plastic Surgery
- Publication Type:Journal Article
- Keywords:
Cell culture;
Microencapsulation;
NIH3T3 cells;
Vascular endothelial growth factor
- From:
Academic Journal of Second Military Medical University
2010;29(5):491-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare NIH3T3 cells harboring microencapsulated VEGF gene and investigate the proliferation, activity and metabolic function of the modified cells. Methods: Microencapsulated VEGF modified NIH3T3 cells were prepared through an alginate-BaCl2 process. Morphological appearance of the microencapsulation and the cell morphology were observed under inverted phase microscope; untreated NIH3T3 cells served as control. The concentrations of VEGF in the culture supernatant (collected every 48 hours) were measured by ELISA; the proliferation and vitality of the cells were examined by MTT assay and flow cytometry with PI staining. Results: The microcapsules were round in shape and the cells grew well. There was no significant difference in the concentrations of VEGF,MTT values and vitalities of cells between the 2 groups. Conclusion: The growth and metabolic functions of NIH3T3 cells are not influenced by microencapsulated NIH3T3 cells harboring VEGF gene. The bio-properties of modified cells are similar to those of the control cells,which lays a foundation for transplantation of microencapsulated VEGF modified NIH3T3 cells in vivo.
