Silence of Wnt5a gene inhibits migration and invasion of lung squamous carcinoma cells

Author: Wei-Xing WANG ¹
Author Information

1. Department of Radiology
Publication Type:Journal Article
Keywords: Cell migration; Lung neoplasms; Neoplasm invasiveness; RNA interference; Squamous cell neoplasms; Wnt5a
From: Academic Journal of Second Military Medical University 2010;29(6):626-629
CountryChina
Language:Chinese
Abstract: Objective: To study the influence of Wnt5a gene silence on the proliferation, migration and invasion of lung squamous carcinoma cells. Methods: A recombinant plasmid pH1-siRNAWnt5a- was constructed and used to deliver small interference RNA (siRNA) targeting Wnt5a in SK-MES-1 cells; the transfected cells were screened to establish a stable transgenic cell line. MTT, cell cycle and Transwell assays were employed to evaluate the effect of Wnt5a gene silence on the proliferation, migration and invasion of lung squamous carcinoma cells. Results: Western blotting assay revealed that Wnt5a was lowly expressed in SK-MES-1Wnt5a- (13.6%). The proliferation index (PI) of transgenic cell line was slightly lower than that of the control cell line ([28.3±3.8] % vs [30.5±5.2]%). The migration and invasion capabilities of SK MES-1Wnt5a- cells were (47.3±9.2)% and (39.7±11.7)% of the control cells, respectively. Conclusion: Low Wnt5a expression can significantly inhibit the migration and invasion capabilities of SK-MES-1 cells, indicating that WntSa might be a potential target for gene therapy of lung squamous carcinoma.