Expression of MMP-9 in brain tissue of mouse cryptococcal meningitis model
10.3724/SP.J.1008.2008.00778
- Author:
Chi-Yu XU
1
Author Information
1. Department of Dermatology
- Publication Type:Journal Article
- Keywords:
Aprotinin;
Cryptococcal meningitis;
Immunohistochemistry;
Matrix metalloproteinase-9
- From:
Academic Journal of Second Military Medical University
2010;29(7):778-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the expression of MMP-9 in mouse brain tissue and its significance. Methods: Cryptococcosis ssuspension alone (fungal suspension group) or combined with aprotinin (aprotinin group) was injected in to female C57 BL/6 mice via femoral vein cannulation. The expression of MMP-9 was examined immunohistochemically 8 h later. Meanwhile, the mouse brain tissue homogenate colony count (CFU) was observed and the results were compared with that of the immunohistochemical examination. Results: The expression of MMP-9 was markedly increased in the fungal suspension group compared with the normal control group (P<0.01); the expression in the aprotinin group was not significantly different from that of the normal control group (P>0.05). The amount of cryptococcosis in the brain tissue was positively correlated with expression of MMP-9. Conclusion: MMP-9 is overexpressed in the brain of mice with Cryptococcal meningitis; MMP-9 might lead to increased permeability of blood brain barrier.
