Promoter region methylation analysis of glutathione S-transferase p1 gene in prostate carcinoma
10.3724/SP.J.1008.2008.00762
- Author:
Wen-Bin DU
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Glutathione transferase;
Methylation;
Promoter regions;
Prostatic neoplasms
- From:
Academic Journal of Second Military Medical University
2010;29(7):762-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the promoter region CpG island methylation status of glutathione S-transferase p1 (GSTP1) gene in prostate carcinoma (PCa) tissues and to search for new molecular markers for the early diagnosis of PCa. Methods: The promoter region CpG island methylation status of 31 PCa, 18 benign prostatic hyperplasia tissues (BPH) and 3 normal prostate tissues (NP) were examined by nest methylation-specific PCR (NMSP) technique, cloning and sequencing. Results: NMSP showed that the methylation rates of PCa, BPH and NP tissues were 83%, 0% and 0%, respectively. The CG sites methylation rates of PCa, BPH and NP tissues were 96%, 34% and 37%, respectively as determined by cloning and sequencing (P<0.01), with no significant difference found between those of BPH and NP tissues(P>0.05). A combination of NMSP and prostate-specific antigen (PSA) was better than PSA alone in screen of PCa. Conclusion: CpG islands of promoter region of GSTP1 gene is hypermethylated in PCa tissues. NMSP is sensitive and specific in detection of GSTP1 methylation, which may serve as a new method for early screen of prostate carcinoma.
