Oxidative stress and intimal hyperplasia lesion in vascular tissues after vascular adventitial injury
10.3724/SP.J.1008.2008.00912
- Author:
Yue-Xia TANG
1
Author Information
1. The First Sanitorium of Qingdao
- Publication Type:Journal Article
- Keywords:
Adventitial;
Atherosclerosis;
NADPH oxidase;
Oxidative stress;
Reactive oxygen species
- From:
Academic Journal of Second Military Medical University
2010;29(8):912-916
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To assess the role of NADPH oxidase-related oxidative stress in the intimal hyperplasia induced by adventitial injury. Methods: Animal model of vascular adventitial injury was established by combining collogenase digestion and mechanical dissection. HE staining was used to observe the morphological changes of the vessels after adventitial injury; RT-PCR was used to examine the mRNA expression of NADPH oxidase subunit p22phox and antioxidant heme oxygenase-1; and fluorescence probe was employed to detect the ROS production in the vessels. Results: Adventitial injuries could induce intimal hyperplasia lesions in the vessels; they also led to the elevated expression of p22phox/HO-1 mRNA and increased production of ROS. Conclusion: The vascular adventitia is involved in the pathological process of intimal hyperplasia, and oxidative stress caused by increased NADPH oxidase activity may be one of the mechanisms.
