Attachment of Human Gingival Fibroblasts to Commercially Pure Titanium Surfaces with Different Instruments: A comparative Study in Vitro.
10.5051/jkape.1999.29.3.607
- Author:
Sung Chan SEO
1
;
In Taeck SONG
;
Jeong Su LIM
;
Hyung Seop KIM
Author Information
1. Department of Periodontology and Research Institute of Oral Bio-science, College of Dentistry, Chonbuk National University, Korea.
- Publication Type:Comparative Study ; In Vitro ; Original Article
- MeSH:
Cell Count;
Fibroblasts*;
Humans*;
Plastics;
Stainless Steel;
Stroke;
Titanium*
- From:The Journal of the Korean Academy of Periodontology
1999;29(3):607-619
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study examined the human fibroblasts cell attachment to commercially pure titanium surface which had been instrumented by 3 types of periodontal instruments. Commercially pure titanium plates were uniformly scaled using plastic, stainless steel, titanium curette. these all experimental groups 65 undirectional strokes with the designated curettes. Alteration of the surfaces due to instrumentation was evaluated by Form Talysurf(R) and reported as Ra value(mean surface roughness). Then other experimental groups were immersed in a cell suspension of human gingival fibroblasts(1x10(5) cell/ml). After 3 days of culture, cell attachment and morphology was observed by SEM, and attached cell were counted by Hemocytometer. A significant difference in mean Ra value was observed for surface instrumented by metal curette compared to either control surface or surface instrumented by the plastic curette(P<0.01). No stastically significant difference was noted between control surface and those instrumented by the plastic curette. SEM observation showed that cell morphology and attachment to the commercially pure titanium plate was similar appearance on the all experimental groups. Experimental groups instrumented by titanium curette and stainless steel curette were more attached cell number than control group, but experimental group instrumented by plastic curette were similar with control groups(P<0.01). In summary, metal curette produced an significant alteration of the commercially pure titanium surface and more favorable surface topography for cell attachment. Otherwise plastic curette was insignificantly altered the commercially pure titanium surface(P<0.01).
