Effects of simvastatin on renal expression of Fas/Fas-L in aged diabetic rats after uninephrectomy
10.3724/SP.J.1008.2008.01046
- Author:
Lan LUO
1
Author Information
1. Department of Geratology
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Fas/Fas-L;
Real-time quantitative polymerase chain reaction;
Simvastatin
- From:
Academic Journal of Second Military Medical University
2010;29(9):1046-1051
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of hydroxy-methyl-glutaryl coenzymeA reductase inhibitor (HMG-CoA RI) simvastatin on renal expression of apoptosis related gene Fas/Fas-L in aged diabetic rats after uninephrectomy. Methods: Elderly uninephrectomized SD rats were randomly divided into uninephrectomized control group (group C), streptozocin(STZ)-induced diabetic model group(group D), and diabetes + simvastatin group (group S). The apoptosis indices in renal tisses of three groups were detected by terminal deoxynucleotide mediated nick end labeling 12 weeks later. Immunofluorescence and real-time quantitative PCR were employed to observe the expression changes of Fas/Fas-L genes at the transcription and translation levels. Results: Compared with group C, group D had significantly higher apoptosis index and expression level of Fas/Fas-L; the above parameters were lower in group S compared with those in group D (P<0.05). The expression of Fas/Fas-L protein was mainly in the renal tubules. Conclusion: Both Fas and Fas-L genes are associated with the development and progression of diabetic nephropathy in aged rats. Simvastatin can down-regulate the translation and transcription of the genes, inhibit cell apoptosis and protect the kidney.
