Study on the Apoptosis and its Mediators in the Placentas of Preclampsia using cDNA Expression Array.
- Author:
Won Jun CHOI
1
;
Wan Sung CHOI
;
Jong Hak LEE
;
Won Young PAIK
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National University, Chinju, Korea.
- Publication Type:Original Article
- Keywords:
Apoptosis;
Peclampsia;
Placenta;
cDNA expression array
- MeSH:
Apoptosis*;
Caspase 10;
Cell Division;
Cyclin A;
Deoxyuridine;
DNA, Complementary*;
In Situ Nick-End Labeling;
Insulin-Like Growth Factor Binding Protein 3;
Placenta*;
Pre-Eclampsia;
Trophoblasts;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Obstetrics and Gynecology
2003;46(2):259-267
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The present study was designed to examine whether preeclampsia is associated with an increase in placental apoptosis and to detect differential expression of mediators involved in apoptosis. METHODS: Placental tissues from 10 cases of preeclampsia and 16 cases of control were analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining. And cDNA expression array assay was performed for detecting mediators that were involved in placental apoptosis. RESULTS: In TUNEL staining, number of apoptotic nuclei were increased in placenta of preeclampsia. The expression of apoptotic signals was limited to trophoblasts. As a result of cDNA expression array assay, expressions of caspase-10, tumor necrosis factor receptor-1 (TNFR-1), and death domain receptor-3 (DDR-3) were increased and expression of cell division protein kinase-9 (CDK-9), cyclin A, insulin-like growth factor-1 (IGF-1), and insulin-like growth factor-binding protein-3 (IGFBP-3) were decreased in the placenta of preeclampsia. The expression of caspase-10, DDR-3, and IGFBP-3 between preeclampsia and normal revealed significantly different (p<0.05). CONCLUSION: Apoptosis was significantly increased in placenta of preeclampsia and various mediators were involved in apoptotic mechanism. Therefore, it was suggested that apoptosis might play a role in the pathophysiologic mechanisms of preeclampsia.
