Effects of composite Chitosan/Heparin coated membrane through a layer by layer self-assemble technique on stent thrombosis
10.3724/SP.J.1008.2008.01324
- Author:
Wen YU
1
Author Information
1. Department of Cardiology
- Publication Type:Journal Article
- Keywords:
Chitosan;
Heparin;
Self-assemble;
Stents;
Thrombosis
- From:
Academic Journal of Second Military Medical University
2010;29(11):1324-1327
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence of the composite chitosan/ heparin coated membrane through a layer-by-layer self-assemble technique on stent thrombosis. Methods: The composite chitosan/heparin coated membrane, which was assembled through a layer-by-layer technique,was attached to the inside surface of cylinder made from 316L stainless steel flake to form membranes of different layers of chitosan/heparin. The activated partial thromboplastin time (APTT),prothrombin time (PT) and thrombin time (TT) of the whole blood samples of healthy controls were examined by clotting method; and the stability of its anticoagulation effect was also examined. The influence of the prepared composite chitosan/heparin coated membrane on coronary stent thrombosis was investigated using swine model of arteriovenous shunt thrombus. Results: The APTT and TT were significantly longer in the composite coated membrane group compared with those of 316L stainless steel group (P < 0.01). Moreover, the prolongation of APTT and TT was correlated with the increase of coated layers. The anticoagulant function of chitosan/heparin coated membrane was stable for two weeks(P>0.05). The weight of thrombus was significantly lower in the coated stent group than that in 316L stainless steel group(P<0.001). Conclusion: The composite chitosan/heparin coated membrane made by layer-by-layer self-assemble has effective and stable anti-thrombotic function.
