Establishment of androgen-independent human prostate cancer line LNCaP by gradual deprivation of hormone
10.3724/SP.J.1008.2008.01311
- Author:
Jia-Tao JI
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Androgen-independent;
LNCaP cell line;
Prostatic neoplasms
- From:
Academic Journal of Second Military Medical University
2010;29(11):1311-1315
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish and identify androgen-independent human prostate cancer cell line LNCap by culturing LNCaP cells with gradual deprivation of hormone. Methods: LNCaP cells were cultured in the medium with gradual deprivation of hormone (treated by active carbon to simulate androgen deprivation) for 10 days; and then the cells were cultured with complete deprivation of androgen for 3 months till the cell entered the rapid proliferation phase again. The cell growth and expression of PSA and androgen were examined by CCK-8, immunfluorescence and RT-PCR methods. Results: LNCaP cells grew slowly after deprivation of hormone and took on a neuroendocrine phenotype and cluster growth pattern. After 3 months' non-androgen culture,the cells regained original morphology and growth. CCK-8 indicated that LNCaP cells could grow in non-androgen condition; immunofluorescence assay indicated that LNCaP-AI cells could regain PSA-secreting activity in non-androgen condition; and RT-PCR suggested that androgen was highly expressed in LNCaP-AI cells. Conclusion: Androgen-independent LNCaP cell line can be established by culturing with gradual deprivation of hormone for 3 months.
