Clinical Significance of Apoptotic Rates of Human Luteinized Granulosa Cells in In Vitro Fertilization and Embryo Transfer.
- Author:
Eun Kyong KIM
1
;
Seok Hyun KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
IVF-ET;
Luteinized granulosa cells;
Apoptosis;
Apoptotic rate;
Pregnancy;
Endometriosis
- MeSH:
Apoptosis;
Embryo Transfer*;
Embryonic Structures*;
Endometriosis;
Estradiol;
Female;
Fertilization in Vitro*;
Granulosa Cells*;
Humans*;
In Situ Nick-End Labeling;
Infertility;
Lutein*;
Oocyte Retrieval;
Pregnancy;
Sensitivity and Specificity
- From:Korean Journal of Obstetrics and Gynecology
2003;46(2):296-305
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the clinical significance of apoptotic rates of human luteinized granulosa cells in in vitro fertilization and embryo transfer (IVF-ET) program. MATERIALS AND METHODS: From 62 infertile patients undergoing controlled ovarian hyperstimulation (COH) for IVF-ET, luteinized granulosa cells were isolated during oocyte retrieval. The apoptotic rates of luteinized granulosa cells were measured by immunocytochemical staining with TUNEL method, and compared with the various clinical parameters. RESULTS: The apoptotic rates were not significantly different among the groups with the various etiologic factors of infertility. The presence of endometriosis, as well as the extent of endometriosis, did not show any differences in the apoptotic rates. The apoptotic rates were negatively correlated with the embryo quality and serum estradiol levels on hCG day. The apoptotic rates of 2.92% or below in the luteinized granulosa cells could predict a successful pregnancy with the sensitivity of 38.9% and the specificity of 93.2%. CONCLUSION: The apoptotic rates of human luteinized granulosa cells may be used to predict the outcome in IVF-ET program.
