Screening and identification of two Macrolactin S-producing bacteria from the sea
10.3724/SP.J.1008.2009.00024
- Author:
Wen-Ju HAN
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- Keywords:
16S rDNA;
Bacillus sp.;
Identification;
Macrolactin S
- From:
Academic Journal of Second Military Medical University
2010;30(1):24-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate and screen active marine microorganisms from the East China Sea and to identify the phenotypes of the isolated strains. Methods: Thirty strains of bacteria isolated from the East China Sea were selected randomly, and the active strains were screened out by the anti-Escherichia coli model. The active strains were subjected to physiological and biochemical characteristics, salt-aggregation test, and 16S rDNA sequence analysis. Neighbor-joining trees were constructed by comparing the results of 16S rDNA sequences with sequences described in the BLAST server of the National Center for Biotechnology Information (NCBI); the strains were subsequently identified to genus level. Results: Strains F81612 and F201721 were screened out with the optimum salinities of 10% and 7.5%, respectively. Their morphology and biochemical characteristics were similar to those of Bacillus sp.. 16S rDNA sequence analysis showed that sequences of F81612 had a higher similarity to those of Bacillus subtilis; F201721 was similar to Bacillus amylolique faciens. Conclusion: Two Macrolactin S-producing strains have been screened out by the anti-Escherichia coli model, and they are identified as moderate halophilic Bacillus sp.
