Preparation and characterization of Holothuria nobilis saponins nobilisideA freeze-dried liposome
10.3724/SP.J.1008.2009.00202
- Author:
Dan GUO
1
Author Information
1. Department of Pharmaceutics
- Publication Type:Journal Article
- Keywords:
Entrapment efficiency;
Freeze-drying;
Liposomes;
NobilisieA;
Stability
- From:
Academic Journal of Second Military Medical University
2010;30(2):202-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare and characterize nobilisideA freeze-dried liposome. Methods: The nobilisideA liposomes were prepared using a film dispersion method followed by a freeze-dried technique. The freeze-dried liposomes were characterized with regard to the drug content, entrapment efficiency, the particle size, ζ potential, and its stability and hemolytic rate in glucose transfusion. Results: Through optimized technique, the prepared nobilisideA freeze-dried liposome, with sucrose as protective agent, had satisfactory structure and redissolubility. The Contents of nobilisideA liposome before and after freeze-drying were 108.3% and 108.6%, the entrapment efficiency were 98.4% and 97.8%, the particle sizes were 89.9 nm and 115.8nm, and the ζ potentials were -19.9mV and -15.9mV, respectively. The liposome content, entrapment efficiency, particle size and hemolytic rate were stable within 8 h during glucose transfusion. Conclusion: NobilisideA freeze-dried liposomes have a narrow size distribution range, a high entrapment efficiency and a stable character, making it a promising candidate for nobilisidesA liposome.
