HPLC simultaneously determines multiple hydrophilic and lipophilic bioactive components in Radix et Rhizoma Salviae Miltiorrhizae
10.3724/SP.J.1008.2009.00190
- Author:
Zi-Yang LOU
1
Author Information
1. Center of Drug Analysis and Testing
- Publication Type:Journal Article
- Keywords:
Clyptotanshinon;
High pressure liquid chromatography;
Multicomponent assaying;
Radix et Rhizoma Salviae Miltiorrhizae;
Rosmarinic acid;
Salviamolic acid B;
Tanshinone II A
- From:
Academic Journal of Second Military Medical University
2010;30(2):190-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a new method for the simultaneous determination of two hydrophilic components and two lipophilic components of Radix et Rhizoma Salviae Miltiorrhizae. Methods: The HPLC-DAD method was employed using a column of Agilent Zorbax TC C18 (4.6 mm × 250 mm, 5 μm) with a mobile phase of methanol -2% acetic acid. The gradient elution program was as follow:0-15 min, 30% B-40% B; 15-20 min, 40% B-60% B; 20-25 min, 60% B-90% B; 25-40 min, 90% B. The detection wavelength was set at 281 nm and the temperature was 35°C. Results: The linearity was obtained over 3.76-120.20 μg · ml-1 (r=0.999 9) for rosmarinic acid, 34.20-109 4.5 μg · ml-1 (r=0.999 9) for salviamolic acid B, 0.64-20.32 μg · ml-1 (r=0.999 9) for clyptotanshinon, and 1.02-32.72 μg · ml-1 (r=0.999 6) for tanshinone II A. The RSDs of precision and stability of the sample were both less than 1% in 48 hours. The average recovery was between 99.72%-100.63%. Conclusion: The present method is simple and has satisfactory efficacy; it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.
