Effect of Cytochalasin B on Survivability and in Vitro Development of Mouse Oocyte Frozen by Vitrification.
- Author:
Won Jun CHOI
1
;
Soon Ae LEE
;
Jong Hak LEE
;
Won Young PAIK
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National University, Jinju, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Mouse oocyte;
Cytochalasin B;
Vitrification;
Survivability;
Development rate
- MeSH:
Animals;
Blastocyst;
Cytochalasin B*;
Cytoskeleton;
Embryonic Development;
Female;
Fertilization;
Mice*;
Nitrogen;
Oocytes*;
Pregnancy;
Sucrose;
Vitrification*
- From:Korean Journal of Obstetrics and Gynecology
2003;46(2):317-322
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this study was to evaluate the effect of CCB treatment on the survivability and in vitro development of mouse oocyte frozen by vitrification. METHODS: Mouse oocytes retrieved from cycle stimulated by PMSG and hCG were treated by CCB and then exposed to EFS-30. These oocytes were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing of the oocytes on EM grid was carried out at room temperature for 5 seconds, then the EM grid was placed into 0.75 M sucrose at 37degrees C for 3 minutes. This was followed by 0.5 M and 0.25 M sucrose for 3 minutes, each. We compared the survivability, cleavage and developmental rate of mouse frozen by vitrification between CCB treated and non-treated groups. Chi-square was used to determine statistical significance. statistical significance was defined as p<0.05. RESULTS: Survivability (79.3%) and developmental rate into blastocyst (52.3%) of mouse oocyte were markedly decreased after vitrification. There were no significant differences between CCB treated and non- treated groups regarding survivability of oocyte frozen by vitrification (80.3% vs 78.5%). The developmental rate into 2-cell in CCB treated group was significantly higher than that in non-treated group (69.7% vs 61.9%, p<0.05). The developmental rate into blastocyst in CCB treated group was higher than that in non-treated group (54.9% vs 51.5%), but the difference was not significant. CONCLUSION: Survivability of mouse oocyte could not be affected by CCB treatment and developmental rate into 2-cell was improved in CCB treated group. It is suggested that CCB treatment prior vitrification improve stability of cytoskeleton and then improve fertilization and early stage embryo development.
