Explant combined with trypsin-digestion for culture of endothelial cells from infantile hemangiomas
10.3724/SP.J.1008.2009.00147
- Author:
Hong-Yue LIAO
1
Author Information
1. Department of Plastic Surgery
- Publication Type:Journal Article
- Keywords:
Cell culture;
Endothelial cells;
Explant combined with trypsin-digested technique;
Hemangioma;
Infantile
- From:
Academic Journal of Second Military Medical University
2010;30(2):147-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore a novel method for cultivation of the endothelial cells(EC) from infantile hemangiomas (IH) in vitro and observe the biological character of cultured endothelial cells. Methods: Fresh operative specimens were obtained from infantile hemangiomas. The endothelial cells of IH were cultured by explant combined with trypsin-digestion technique. Immunohistochemical staining of EnVision method was carried out to identify the cultured cells. The purity of endothelial cell was examined by flow cytometry analysis of FITC-CD34. The biologic characters of endothelial cells were observed under inverted phase contrast microscope. Results: Endothelial cells were successfully cultured from 8 IH specimens of all the 14 explants. The morphology of cultured endothelial cells included two types:polygonal cells and fusifourm cells. The cultured cells were homogenously positive for EC markers-vWF or CD34, indicating their EC origin. Tube formation was found in endothelial cells of 1H. The proportion of CD34+ cells was 76.28% in cultured endothelial cells as detected by flow cytometry analysis. Conclusion: ECs can be successfully isolated and cultured from infantile hemangiomas by explant combined with trypsin-digestion technique; the cultured cells have some characters of endothelial cells.
