Regulatory mechanism of transforming growth factor-beta 1 on mechanical properties of tissue engineered heart valve
10.3724/SP.J.1008.2009.00124
- Author:
Hao HONG
1
Author Information
1. Department of Cardiovascular Surgery
- Publication Type:Journal Article
- Keywords:
Collagen;
Heart valve;
Lysyl oxidase;
Mechanical properties;
Tissue engineering;
Transforming growth factor-beta 1
- From:
Academic Journal of Second Military Medical University
2010;30(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe whether transforming growth factor-beta 1 (TGF-β1) can improve the mechanical properties of tissue engineered heart valve (TEHV) prepared by TGF-β1 and myofibroblasts. Methods: Myofibroblasts were isolated, cultured, identified, and seeded onto the decellularized aortic valve leaflet. In the experimental group, TEHV were cultured with DMEM containing 10 ng/ml TGF-β1 for 14 days. In the control group, TEHV were cultured with DMEM only for 14 days. The contents of lysyl oxidase (LOX) and hydroxyproline, the expression of LOX and COLL-1 mRNA, and the mechanical properties of the TEHVs were analyzed in each group. Results: The contents of LOX and hydroxyproline, expression of LOX and COLL-1 mRNA, Max-load, and Max-stress were significantly higher in the experimental group than those in the control group (all P<0.05). However, the experimental and control group showed comparable values of Max-strain and elastic modulus. Conclusion: TGF-β1 can increase expression of LOX and COLL-1 mRNA in the seeded cells during TEHV construction, increasing the production and cross-linking of collagen, and improve the mechanical properties of the TEHV.
