mRNA expression of BCSG1, BTF, Bcl-2, Erα and ERβ in osteosarcoma and its significance
10.3724/SP.J.1008.2009.00416
- Author:
Ming LEI
1
Author Information
1. Department of Orthopedics
- Publication Type:Journal Article
- Keywords:
Bcl-2;
Beast carcinoma specific gene;
Estrogen receptor;
Osteosarcoma;
Transcription factors
- From:
Academic Journal of Second Military Medical University
2010;30(4):416-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the mRNA expression of BCSG1 (breast carcinoma specific gene, BCSG1), BTF(Bcl-2-associated transcription factor, BTF), Bcl-2 (B cell lymphoma/leukemia-2, Bcl-2) and two subtypes of estrogen receptors in osteosarcoma and their implication. Methods: The expression of BCSG1, ERα, BTF, ERβ, and Bcl-2 mRNA were examined in 16 poorly differentiated osteosarcoma samples and a cell line hFOB 1.19 by quantitative RT-PCR. The relationship between their expression was analyzed. Results: The mRNA expression of BCSG1, Bcl-2 and two subtypes of estrogen receptors in the osteosarcoma tissues was significantly higher than that in the osteoblast cell line of hFOB 1.19; the expression of BTF was not significantly different. ERα/ERβ in the osteosarcoma tissues was significantly higher than that in the hFOB 1.19 cells (P< 0.01). BCSG1, ERα, ERβ, Bcl-2 and BTF expression was not associated with the tumor size or patients gender. Correlation analysis showed there was a linear relationship between expression of BCSG1 and ERα, ERβ and Bcl-2, and ERα/ERβ ratio and Bcl-2. Conclusion: BCSG1 may enhance the activation of estrogen receptor-α gene transcription, and ERβ may influence the expression of Bcl-2 in osteosarcoma.
