Effect of hyperbaric oxygen on number of peripheral blood stem cells: Clinical observation and experimental study
10.3724/SP.J.1008.2009.00387
- Author:
Cheng-Gang ZHENG
1
Author Information
1. Hyperbaric Oxygen Medical Center
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell;
Hyperbaric oxygenation;
Mobilization;
Peripheral blood
- From:
Academic Journal of Second Military Medical University
2010;30(4):387-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of hyperbaric oxygen (HBO) on peripheral hematopoietic stem cells (PHSCs) in a Chinese population, and to mobilize PHSCs by combined use of HBO and colony-stimulating factor (CSF) in an animal experiment. Methods: (1) The changes of CD34+ absolute count before and after HBO therapy were observed under routine HBO conditions in 8 patients who received long-term HBO therapy and 18 patients who received HBO therapy for neurological injuries. (2) Thirty BALB/c mice were equally randomized into three groups: HBO group, where the animals were exposed to HBO; HGF group, where the animals received recombinant hematopoietic growth factor injection; and HBO+ HGF group. Postglobe venous blood was harvested before and after treatment; the gated cell CD34/Scal-1 dual-positive population was determined by flow cytometry. Results: (1) After 7 episodes of HBO therapies, the peripheral CD34+ absolute count of the patients rose from (1.87 ± 1.08) × 106/L before treatment to (4.75 ± 4.67) × 106/L (an increase of 2.54 folds); while the number was (2.85 ± 1.94) × 106/L after 14 episodes of HBO therapies (with an increase of 1.52 folds) and (3.26 ± 3.49) × 106/L after 20 episodes of HBO therapies (with a increase of 1.74 folds). The peripheral CD34+ absolute count of the 8 patients who received long-term HBO therapy for neurological injuries was (2.11 ± 1.14) × 106/L, not significantly different from that of the 18 patients before treatment. (2) The gated cell CD34/Scal-1 dual-positive population in HBO group, HGF group, and HBO+HGF group after treatment were 2.01, 2.66, and 3.76 folds before treatment, respectively. Conclusion: Multiple HBO exposure can mobilize PHSC; prolonged HBO exposure fails to maintain the peripheral CD34+ absolute count at a relatively high level. Concomitant use of HBO and HFG can more effectively mobilize PHSCs.
