Anti-apoptosis effect of tremella polysaccharides on cardiomyocytes
10.3724/SP.J.1008.2009.00383
- Author:
Dan QU
1
Author Information
1. Department of Nutrition
- Publication Type:Journal Article
- Keywords:
Antioxidation;
Apoptosis;
Cardiomyocyte;
Tremella polysaccharides
- From:
Academic Journal of Second Military Medical University
2010;30(4):383-386
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the anti-apoptosis effect of tremella polysaccharides (TP) on cardiomyocytes by in vitro and in vivo experiments. Methods: TP was extracted by dehydrated alcohol and the TP purity was measured by gas chromatography. The cultured cardiomyocytes of the neonatal rats were randomly assigned to normal group (Group A), apoptosis-induced group (Group B) and TP-pretreatment group (Group T). The morphology and uptake rate of trypan blue were studied after 72 h. The apoptosis index of cardiomyocyte was measured by flow cytometry. In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following: Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks. Results: Data of cultured cardiomyocytes were as follows: Uptake rate of trypan blue: B>C>A (P<0.01); apoptosis index: B>C> A (P<0.01). In the animal studies there were no obvious difference in myocardium pathology between groups. The order of the apoptosis indices of myocardium was H
