Influence of RNA interference targeting P13K p85a expression on invasion and metastasis of colorectal cancer cells
10.3724/SP.J.1008.2009.00496
- Author:
Yan SUN
1
Author Information
1. Institute of Gastroentcrology
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Neoplasm metastasis;
P13K p85α;
RNA interference
- From:
Academic Journal of Second Military Medical University
2010;30(5):496-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the infloence of RNA interference targeting P13K p85α on the invasion and metastasis of colorectal cancer cells. Methods:Foor shRNA vectors and one negative control vector were designed and stably transfected into LoVo cells. Western blotting analysis was used to screen for the groop which had the highest inhibitory rate; the cells were sobjected to woond closore assay and transwell assay to determine the migration of LoVo cells after stable transfection. Results: Western blotting analysis showed that cells transfected with shRNA/324 vector had the strongest inhibition of P13K p85α protein, with the inhibition rate being 77% , and cells in this groop were osed for woond closore assay and transwell assay. Wound cloaore assay showed that the healing ability of P13K p85α-depleted cells was significantly lower than that of the control cells (P<0.05). Transwell assay showed that the cells passing the polycsrhonate membranes was significantly less in P13K p85α-knockdown cells than in the control cells (P<0.05). Conclusion:Depletion of P13K p85α protein can obvioosly inhibit the migration of LoVo cells. P13K p85α may be a new therapeotic target for treatment of colorectal cancer metastasis.
