The role of Interferon-gamma on the Expression of Class I Human Leukocyte Antigens in a Human First Trimester Trophoblast Cell Line.
- Author:
In Bai CHUNG
1
;
Hey Kyoung HAN
;
Na Ok KIM
;
Jung Sik HA
;
Hyun Sook PARK
;
Joo Young PARK
Author Information
1. Department of Obstetrics and Gynecology, Yonsei University, Wonju College of Medicine, Wonju, Kangwon-do, Korea.
- Publication Type:Original Article
- Keywords:
Trophoblast;
Human Leukocyte Antigen;
Interferon-gamma
- MeSH:
Abortion, Habitual;
Abortion, Spontaneous;
Cell Line*;
Cytokines;
Female;
Fetus;
HLA Antigens*;
HLA-C Antigens;
Humans*;
Interferon-gamma*;
Polymerase Chain Reaction;
Pregnancy;
Pregnancy Trimester, First*;
Reverse Transcription;
Trophoblasts*;
United Nations
- From:Korean Journal of Obstetrics and Gynecology
2003;46(2):378-386
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Precise mechanism which the fetus can escape from mother's immune rejection is not well understood yet during last 50 years. The clarification of immune mechanism at the feto-maternal interface is very important, because this can be a common pathogenesis of various pathologic conditions including spontaneous abortion and habitual abortion. The pivot of this mechanism is cross-talk between the expression of HLA-C, E, G on the extravillous cytotrophoblasts and their receptors on decidual NK (natural killer) cell. The mechanism through which HLA-C, E, G regulation on the extravillous trophoblast is poorly understood. The IFN-gamma is known to be harmful to the successful maintenance of early pregnancy. OBJECTIVE: To clarify whether the IFN-gamma could be involved in the regulation of the HTR-8/SVneo human first trimester cell line. METHODS: We investigated the effects of IFN-gamma only or with other cytokines on the HLA-C, E, G transcripts in human first trimester trophoblast cell-line by using Reverse Transcription Polymerase Chain Reaction (RT-PCR). RESULTS: The trophoblast cells expressed HLA-C, E constitutively. When cultured in the presence of IFN-gamma only or with other cytokines, HLA-C, E transcripts were not significantly affected. CONCLUSION: These results suggest that harmful IFN-gamma to maintain early pregnancy may affect through another mechanism besides regulation of HLA-C, E on the extravillous cytotrophoblasts at the feto-maternal interface.
