Selection of peptides bind to PreS1 antigen from a phage-displayed peptide library
10.3724/SP.J.1008.2009.00783
- Author:
Qing-Qing CHEN
1
Author Information
1. Department of Biochemical Pharmacology
- Publication Type:Journal Article
- Keywords:
Binding peptide;
Hepatitis B;
Phage display peptide library;
Pres1 antigen
- From:
Academic Journal of Second Military Medical University
2010;30(7):783-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library. Methods: The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph. D. -7 peptide library with phage display technique, and the positive clones were identified by ELISA. Results: After three rounds of biopanning, the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA. Sequencing result showed that the binding peptides had high affinity and specificity. Conclusion: A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library, which paves a way for the diagnosis and treatment of hepatitis B infection.
