HPLC-TOF/MS in rapid identification of chemical compositions in Xiaochaihu decoction
10.3724/SP.J.1008.2009.00941
- Author:
Xiao-Fan LIU
1
Author Information
1. Department of Drug Analysis
- Publication Type:Journal Article
- Keywords:
Chemical principles(TCD);
HPLC-TOF/MS;
Xiaochaihu decoction
- From:
Academic Journal of Second Military Medical University
2010;30(8):941-946
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To rapidly identify the chemical components in a traditional Chinese herb Xiaochaihu decoction by high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods: An Agilent ZORBAX SB-C 18 column (100 mmX3.0 mm,3.5 μm) was used for rapid separation and identification of chemical components in Xiaochaihu decoction, with a mobile phase of methanol and 0.1% formic acid aqueous solvent in gradient elution. The flow rate was 0.6 ml/min and the injection volume was 4 μl. The detector was time-of-flight mass spectrometer with an ESI ion source. Scanning mass range was m/z 200-1 500. Results: Thirty-eight chemical compounds were identified from Xiaochaihu decoction in one run. Conclusion: Thirty-eight chemical compounds have been identified in Xiaochaihu decoction by HPLC-TOF/MS in one run, which paves a way for further understanding the metabolism and mechanism of the chemical compounds in Xiaochaihu decoction.
