Effects of titanium dioxide nanoparticles on development and maturation of rat preantral follicle in vitro
10.3724/SP.J.1008.2009.00869
- Author:
Juan HOU
1
Author Information
1. Department of Health Toxicology
- Publication Type:Journal Article
- Keywords:
Oocytes;
Ovarian follicle;
Titanium dioxide nanoparticles;
Toxicity test
- From:
Academic Journal of Second Military Medical University
2010;30(8):869-873
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of titanium dioxide nanoparticle(TiO 2) on follicle development and oocyte maturation of rats using in vitro rat preantral follicle culture system, so as to know whether TiO 2 can cause female reproductive toxicity and provide evidences for evaluating its safety. Methods: Rat ovaries were mechanically dissected to obtain preantral follicles and the individual preantral follicle was cultured in 96-well plates. The study was divided into 5 groups: three 25 nm TiO 2 groups (12.5 μg/ml, 25 μg/ml and 50 μg/ml), one negative control group (treated with culture medium), and one micron TiO2 control group. Ten days after culture, the ovulation was induced and the follicle development and oocyte maturation were observed. Results: The survival rate of follicles, formation rate of antral follicles and release rate of cumulus-oocyte cell complexes (COCs) all decreased with the increase of 25 nm TiO2 (P<0.05). Compared with the negative control group, micron TiO2 group had no obvious changes in the follicle development and oocyte maturation. Compared with the two control groups, 25 nm TiO2 caused obvious morphological changes of follicles and obvious decrease in follicle survival and formation rate of antral follicles. 50 μg/ml 25 nm TiO2 reduced the number of mature oocytes. Conclusion: 25 nm TiO 2 at 25 μg/ml or above can inhibit rat follicle development and oocyte maturation in vitro, while the micron TiO2 at the same dose has no obvious influence on follicle development and oocyte maturation, suggesting different toxic properties between nmTiO2 and micron TiO2. Titanium dioxide nanoparticle may have female reproductive toxicity and should be given more attention.
