High performance liquid chromatography in determination of chlorogenic acid, baicalin, emodin and chrysophanol contents in Kangjunxiaoyan tablet
10.3724/SP.J.1008.2009.01191
- Author:
Yang FEI
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Baicalin;
Chlorogenic acid;
Chrysophanol;
Emodin;
High performance liquid chromatography;
Kangjun Xiaoyan table
- From:
Academic Journal of Second Military Medical University
2010;30(10):1191-1194
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for determination of chlorogenic acid, baicalin, emodin and chrysophanol contents in Kangjunxiaoyan tablet. Methods: After removing the coating, the powder of Kangjunxiaoyan tablet was extracted by 50% methanol for 30 min using the following condition: analytical column, Agilent Eclipse Plus-C18 column (4.6 mmX250 mm, 5 μm); mobile phase: A, 0.1% (v/v) phosphoric acid-water, B, methanol, C, acetonitrile with gradient elution. The flow rate was 0.8 ml/min; the detection wavelength was set at 254 nm; the temperature of column was 30°C; and the injection volume was 10 μl. Results: The title compounds were separated at baseline within 45 min with good linear, with the regression being Y = 12.89X-12.66, r = 0.9999 for chlorogenic acid, Y = 13.96X-1.013, r=1.0000 for baicalin, Y=35.82X-4.923, r=0.9999 for emodin, and Y=44.16X-3.280, r=0.9999 for chrysophanol. The intra-day and inter-day precisions and the limits of detection were all within the normal range. The recovery rates (n=3) were: chlorogenic acid 101.5% (RSD=1.6%), baicalin 103.4% (RSD=1.4%), emodin 99.3% (RSD=2.0%), and chrysophanol 98.1% (RSD=1.7%). Conclusion: HPLC is a simple, accurate, stable, and reliable method in determining the contents of chlorogenic acid, baicalin, emodin, and chrysophanol in Kangjunxiaoyan tablet, and it can be used for quality control of this preparation.
