Expression of E6 oncoprotein of HPV16, cyclin D1, and human telomerase transcriptase in nasopharyngeal carcinoma tissues and its significance
10.3724/SP.J.1008.2009.01367
- Author:
Li-Zhu JIANG
1
Author Information
1. Department of Otorhinolaryngology
- Publication Type:Journal Article
- Keywords:
Cyclin D1;
E6 oncoprotein of HPV16;
Immunohistochemistry;
Nasopharyngeal neoplasms;
Telomerase transcriptase
- From:
Academic Journal of Second Military Medical University
2010;30(12):1367-1371
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the role of HPV16E6,cyclin D1 ,and human telomerase transcriptase (hTERT) in the development and progression of nasopharyngeal carcinoma ( NPC ) and to discuss the clinical significance. Methods: Immunohistochemistry was used to detect the expression of HPV16E6, cyclin D1, and hTERT in paraffin-embedded nasopharyngeal carcinoma tissues and nasopharyngeal chronic inflammation tissues. The relationship between their expression with the clinicopathological features of NPC was analyzed; the influence of their expression on prognoses of patients was also analyzed. Results: The positive rates of HPV16E6,cyclin D1 ,and hTERT in NPC tissues were 62. 5%(35/56) ,50. 0%(28/56) , and 67. 9%(38/56) ,respectively, which were significantly higher than those in the inflammation tissues (P<0. 05). The positive rates of HPV16E6,cyclin D1 ,and hTERT increased with the decrease of differentiation grades, increase of clinical stage and presence of lymph node metastases ( P < 0. 05), but had no relationship with patients' age or sex(P>0. 05). HPV16E6 expression was positively correlated with cyclin D1(r=0. 480,P<0. 001) and hTERT (r=0. 494,P<0. 001)in NPC tissues. The mean survival period and median survival period in HPV16E6,cyclin D1 and hTERT positive patients were lower than those in the negative ones (P<0. 05). Conclusion: High expression of HPV16E6,cyclin D1 ,and hTERT might be involved in the development and progression of NPC. HPV16E6 may interact with cyclin D1 and hTERT, contributing to the development of NPC. Examination of the three agents may help to predict the prognoses of NPC.
