HPLC in simultaneous determination of four components in Shuangwu capsules
10.3724/SP.J.1008.2010.00091
- Author:
Guang-Ming YE
1
Author Information
1. Departement of Pharmacy
- Publication Type:Journal Article
- Keywords:
Deoxyschizandrin;
High pressure liquid chromatography;
Schisandrin B;
Schizandrin;
Shuangwu capsules;
Syringin
- From:
Academic Journal of Second Military Medical University
2010;31(1):91-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for simultaneous determination of syringin, schizandrin, deoxyschizandrin and schisandrin B in Shuangwu capsules. Methods: The HPLC method was employed using a Diamonsil C18 column (200 mm X 4.6 mm, 5μm) with a mobile phase of methanol-acetonitrile (1 : 1, A) and water (B). The gradient elution program was as follows, 0-5 min, 35%-60%A; 5-10 min, 60%-70%A; 10-50 min, 70%-90%A; 50-90 min, 90%A. The flow rate was 1 ml/min. The detection wavelength was set at 220 nm and the temperature was at 35°C. Results: The linearity was obtained within the range of 1.28-20.40 μg/ml for syringin (r=0.999 7), 6.30-100.80μg/ml for schisantherin (r=0.999 6), 1.20-19.20μg/ml for deoxyschizandrin (r=0.999 8), and 3.75-60. 00 μg/ml for schisandrin B (r=0. 999 6). The RSD values of precision were less than 1% for all the four components. The results showed that the samples were stable in the room temperature for at least 24 h. The average recovery rates of syringin, schizandrin, deoxyschizandrin and schisandrin B were 99.47%, 102.50%, 99.21% and 101.86%, respectively. Conclusion: Our method is rapid, easy to perform and accurate; it can be used to control the quality of Shuangwu capsules.
