HPLC-TOFMS in rapid separation and identification of chemical components in Oldenlandia diffusa and its injection preparations
10.3724/SP.J.1008.2010.00292
- Author:
Wei-Cheng CHEN
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
HPLC-TOFMS;
Identification;
Injection;
Oldenlandia diffusa
- From:
Academic Journal of Second Military Medical University
2010;31(3):292-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective To rapidly separate and identify the chemical components in traditional Chinese herbal medicine of Oldenlandia diffusa and its injection preparations by high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOFMS). Methods An Agilent Zorbax XDB-Q18 column (250 mm×4. 6 mm, 5 μm) was used for separation and identification of chemical components in Oldenlandia diffusa, with a mobile phase of 0. 3% acetic acid (A) and methanol (B) in gradient elution, 0-30 min, 30%-90%B. The flow rate was set at 1. 0 ml/min and the injection volume was 10 μl. The time- of-flight mass spectrometer was equipped with an EIS ion source. The scanning mass range was between m/z 100-1 000. Results The traditional Chinese medicine of Oldenlandia diffusa and its injection preparation were on-line separated and characterized by HPLC-TOFMS, and 11 chemical compounds were identified in Oldenlandia diffusa, 6 compounds in market injection preparation, and 2 compounds in the injection prepared by ourselves. Conclusion Chromatographic demonstration of chemical compounds in Oldenlandia diffusa in one run provides a foundation for the further studying the metabolism and mechanism of Oldenlandia diffusa and its injection preparations.
