NKX6.1 combined with PDX1 induces mesenchymal stem cell differentiation into B-like cells
10.3724/SP.J.1008.2010.00258
- Author:
Xiao-Long TANG
1
Author Information
1. Clinical Laboratory
- Publication Type:Journal Article
- Keywords:
Fetal liver;
Mesenchymal stem cells;
NKX6. 1;
Pancreatic duodenal homeobox 1;
The adenovirus vector
- From:
Academic Journal of Second Military Medical University
2010;31(3):258-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the synergistic effect of NKX6. 1 and PDX1 in inducing differentiation of fetal liver-derived mesenchymal stem cells(FL-MSCs) into the pancreatic B cells and to explore the underlying mechanisms, so as to obtain enough islet-like body for transplantation. Methods Recombinant adenovirus vector harboring both PDX1 and NKX6. 1 genes was constructed, and the vector was used to infect FL-MSCs. Then a series of cytokines were used to induce the differentiation of infected FL-MSCs into pancreatic B cells. The expressions of PDX1, NKX6.1 gene, transcription factors NGN3, NeuroDl/Beta2, MafA as well as C-peptide were examined. Results PDX1 and NKX6. 1 were detected in FL-MSCs cells 24 h after infection; cells began to express NGN3, NeuroDl, and MafA and stably expressed pancreatic B cell related factors including insulin after induction. The expression of these molecules was in a certain order. Conclusion PDX1, NKX6. 1 combined with a series of cytokines can effectively induce FL-MSCs to differentiate into pancreatic islet B cells in vitro, which might be through activation of transcription factors NGN3, NeuroDl, and MafA in turn, inducing FL-MSCs to differentiate towards endocrine precursor cells, B endocrine precursor cells and B cells in turn.
