Effect of siRNA targeting TGF-β inducible early gene on expression of advanced glycation end products-mediated Smad2 in renal tubular epithelial cells
10.3724/SP.J.1008.2010.00354
- Author:
Li-Hong YE
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- Keywords:
Lentiviral vector;
Rna interference;
Smad2;
TIEG;
Tubular epithelial cells
- From:
Academic Journal of Second Military Medical University
2010;31(4):354-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of siRNA targeting TGF-β inducible early gene (TIEG) on expression of Smad2 in advanced glycation end products (AGEs)-mediated renal tubular epithelial cells. Methods: The pshRNA-copGFP-lentivector containing target gene was used as a vector to construct siRNA-TIEG, which was then transfected into normal rat proximal tubular epithelial cells(NRK52E), which were then cultured in RPMI 1640 medium supplemented with AGE-BSA for 24 h and 48 h. The expression of Smad2 mRNA and protein was examined by PCR, Western blotting analysis, respectively. Blank vector group served as control. Results: SiRNA-TIEG significantly reduced the expression of TIEG mRNA, Smad2 mRNA and Smad2 protein in normal rat proximal tubular epithelial cells in presence of AGE-BSA compared with those in the control group (P<0.05). Conclusion: Silence of TIEG can effectively inhibit the expression of Smad2 mediated by AGEs in NRK52E cells.
