Cytotoxic effect of CdSe/ZnS quantum dots on melanoma cells and normal human epidermal cells
10.3724/SP.J.1008.2010.00465
- Author:
Fang-Ming SONG
1
Author Information
1. Center of Drug Analysis
- Publication Type:Journal Article
- Keywords:
CdSe/Zns quantum dots;
Cell proliferation;
Cytotoxicity;
Epidermal cell;
Melanoma
- From:
Academic Journal of Second Military Medical University
2010;31(5):465-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the cytotoxic effect of the CdSe/ZnS quantum dots of different diameters on human malignant melanoma cell line A375, A375. s2 and normal human epidermal cell line HaCaT using cell counting kit-8(CCK-8), so as to study the influence of CdSe/ZnS quantum dots on growth of melanoma cells. Methods: Log phase A375, A375. s2 and HaCaT cells were plated into 96-well plates. After a 24 h incubation, the prepared QDs-605 and QDs-545 solutions of different concentrations (162, 81, 54, 40. 5, 27, 10. 125, 4. 05, 2. 025, and 1. 012 5 nmol/L) were added into the wells. After another 24 h incubation, CCK-8 was added into the well (10 μ1/well). Then the cytotoxicities of CdSe/ZnS quantum dots on the proliferation of A375, A375. s2 and HaCaT cells were determined by MTT assay. Results: We found that with the increase of QDs-605 and QDs-545 concentrations, the survival rates of A375, A375. s2 cells were decreased. A day after A375, A375. s2 cells were treated with different concentrations of QDs-605 and QDs-545, the cell survival rate was significantly lower than that of the control group(P<0.05, P<0.01); however, different concentrations of QDs-605 and QDs-545 did not inhibit the proliferation of HaCaT cells. Conclusion: CdSe/Zns quantum dots have inhibitory effect on the growth of human malignant melanoma cell line A375, A375. s2, and have no noticeable effect on normal human epidermal cell line HaCaT.
