Effect of rosiglitazone and pioglitazone on apoptosis of pancreatic beta-cell in GK rats
10.3724/SP.J.1008.2010.00604
- Author:
Wen-Jun LI
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Insulin-secreting cells;
Pioglitazone;
Rosiglitazone;
Type 2 diabetes mellitus
- From:
Academic Journal of Second Military Medical University
2010;31(6):604-607
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect of two thiazolidiriediones (TZDs), rosiglitazone and pioglitazoneon, on the apoptosis of pancreatic islet beta-cells. Methods: Male Goto-Kakizaki (GK) rats (a spontaneously diabetic animal model of type 2 diabetes mellitus) and non-diabetic Wistar rats were randomly divided into four groups, Wistar group (W group, n=13), GK rats fed with rosiglitazone (R group, n=10), GK rats fed with pioglitazone (P group, n=10) and GK non-treated rats (G group, n=10). Rats in R group were given rosiglitazone (2 mg·kg-1·d-1) by gavage for 6 weeks; those in A group were given pioglitazone (3 mg·kg -1·d-1) by gavage for 6 weeks. The pancreatic specimens were obtained and treated for light microscope and transmission electron microscope. Bcl-2, Bax protein expression was examined by immunohistochemistry. The apoptosis of pancreatic islet beta-cell was examined by TUNEL. Results: The β-cells in G group showed signs of early apoptosis: nuclei shrinkage, disappearing nucleoli, and chromatin aggregation to nuclear membrane, and the changes were not observed in the other three groups. Bcl-2 expression was significantly higher in R group and P group than in G group (P<0.01), and there was no significant difference between the W group and G group. Bax expression was significantly lower in R group, P group, and W group than in G group (P<0.05), and there was no difference between the R group and P group. Conclusion: Increased β-cells apoptosis does exist in GK rat pancreatic islets, which may contribute to the development of diabetes. Rosiglitazone and pioglitazone can prevent β-cell from apoptosis, which might be related to the changes of Bcl-2 and Bax expression.
