Influence of ascorbic acid supplementation on systemic Candidiasis in mice
10.3724/SP.J.1008.2010.00731
- Author:
Hai HUANG
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Ascorbic acid;
Candida albicans;
Systemic fungal infections
- From:
Academic Journal of Second Military Medical University
2010;31(7):731-733
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine the influence of ascorbic acid supplementation on mouse model of Candida albicans infection. Methods: Mouse models of systemic Candida albicans infection were established by the tail vein injection of Candida albicans suspension and were randomly divided into five groups (n= 10). Ascorbic acid was administered by gavage or intraperitoneal injection at a dose of 50 mg/kg or 500 mg/kg. The survival time and renal fungal burden of mice were observed. Renal histopathology specimens were stained by H-E and PAS staining to assess kidney damage. Results: The median survival periods of animals in both ascorbic acid supplementation group and blank control group were 4-5 days. The IgCFU/g values were 8.342, 8.412, 7.350, and 7.764 in experimental groups and 7.134 in the blank control group. Supplementation of ascorbic acid had significant influence on renal fungal burden (P<0.05). Kidney histopathology examination showed renal abscess and renal tubule disorders in mice in all groups, indicating that ascorbic acid supplementation did not alleviate Candida albicans infections. Conclusion: Ascorbic acid supplementation can not improve the condition of systemic Candida albicans infection in mice, and can even exacerbate the condition.
