Effects of osteopontin on proliferation of endothelial progenitor cell in vitro

Author: Yue WANG ¹
Author Information

1. Department of General Surgery
Publication Type:Journal Article
Keywords: Cell proliferation; Endothelial progenitor cells; Osteopontin
From: Academic Journal of Second Military Medical University 2010;31(7):715-720
CountryChina
Language:Chinese
Abstract: Objective: To observe the effect of osteopontin (OPN) on the proliferation of endothelial progenitor cells (EPCs) and to explore the underlying mechanisms. Methods: The total mononuclear cells (MNCs) were isolated from bone marrow by Ficoll density gradient centrifugation, and EPCs were obtained by differential attachment technique from MNCs after culture for 8 days. The EPCs were identified by morphology observation, flow cytometry and test of the ability to intake the acLDL and bind UEA-1. OPN expression in EPCs was examined by immunohistochemistry and RT-PCR. EPC proliferation was assayed by CCK-8 assay after EPCs were treated with different concentrations of OPN or the inhibitors of phosphoinositide 3-kinase (PI3K) and Akt (LY294002 and Akt inhibitor). Results: The recombinant OPN protein increased EPC proliferation in a dose-dependent manner, and inhibition of the PI3K/Akt pathway blocked the influence of OPN on the proliferation of EPCs. Conclusion: Recombinant OPN plays an important role in regulating the in vitro proliferation of bone marrow-derived EPCs via PI3K/Akt pathway.