Types of alternative splice variants of transcription factor 19 and their expression patterns in clear cell renal cell carcinoma tissues
10.3724/SP.J.1008.2010.00706
- Author:
Tao QIAN
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Alternative splicing;
Isoforms;
Kidney neoplasms;
Transcription factors
- From:
Academic Journal of Second Military Medical University
2010;31(7):706-710
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study whether there is expression of the alternative splice variants of TCF19, a cell growth regulatory gene, in human clear cell renal cell carcinoma (ccRCC) tissues and their relationship with the development of ccRCC. Methods: Alternative Splicing Database was used to predict the possible alternative splice variants of TCF19. Specific primers for amplifying the 5 predicted variants of TCF19 were designed and used to examine the corresponding variants in 32 ccRCC tissues and normal tissues adjacent to the tumors by RT-PCR technique. The amplicons were then cloned and sequenced. Quantitative RT-PCR was used to detect the expression differences of the variants between the primary renal cell carcinoma and adjacent tissues. Results: Two new isoforms of TCF19 (TCF19-1 and TCF19-3) were identified in the ccRCC and the adjacent tissues. RT-PCR results showed that TCF19-1 expression was significantly higher in the primary ccRCC tissues than that in the adjacent normal tissues (P<0.001). And the expression of TCF19-3 in the primary tumor was only slightly higher than that in the adjacent tissues (P = 0.082). Conclusion: This study for the first time identifies the existence of two alternative splice isoforms of TCF-19, TCF19-1 and TCF19-3, in human ccRCC tissues, and the expression of TCF19-1 might be related to the development of ccRCC.
