Anti-inflammatory mechanism of selective estrogen receptor modulator in mice with experimental autoimmune encephalomyelitis
10.3724/SP.J.1008.2010.00946
- Author:
Xiao HU
1
Author Information
1. Department of Neurology
- Publication Type:Journal Article
- Keywords:
Experimental autoimmune encephalomyelitis;
Inflammation;
Selective estrogen receptor modulators
- From:
Academic Journal of Second Military Medical University
2010;31(9):946-951
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the anti-inflammatory mechanisms of selective estrogen receptor modulator (SERM) on experimental autoimmune encephalomyelitis(EAE). Methods: EAE models were induced with MOG35-55 peptide in 60 mice and all animals were ovarectomized. The model animals were then divided into treatment group and control group (n = 30). Treatment group was treated with SERM. The clinical symptom scores were compared between the two groups. The pathologic changes of the brain and spinal cord were studied by H-E staining and luxol fast blue(LFB)-HE staining. The expressions of MMP-9, TNF-α, IFN-γ, and IL-4 mRNA and protein were examined by quantitative real-time PCR and ELISA. Western blotting analysis was used to analyze the expression of myelin basic protein (MBP) so as to analyze the demyelination status. Results: Clinical symptom scores and incidence of EAE in the treatment group were improved compared with those in the control group (P<0.01, P<0.05). H-E staining showed that infiltration of inflammatory cells was decreased in the treatment group (P<0.05). LFB-H-E staining and Western blotting analysis showed that the demyelination was improved in the treatment group (P<0.05). The results of quantitative real-time PCR and ELISA showed that the expression of MMP-9,TNF-α, and IFN-γ were decreased and the expression of IL-4 was increased (P<0.05, P<0. 01). Conclusion: SERM can alleviate the inflammation symptom in EAE mice through decreasing MMP-9, TNF-α, and IFN-γ and increasing IL-4 expression.
