Construction of oncogene p28GANK mutants and their regulating effects on p53
10.3724/SP.J.1008.2010.01049
- Author:
Yi-Bin REN
1
Author Information
1. International Co-operation Laboratory on Signal Transduction
- Publication Type:Journal Article
- Keywords:
Mutants;
Oncogene protein p28GANK;
p53 gene
- From:
Academic Journal of Second Military Medical University
2010;31(10):1049-1052
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct the mutants of oncogene p28GANK with different ankyrin repeats deleted, so as to further study the potential role of p28GANK in hepatocellular carcinoma (HCC). Methods: QuikChang Site-Directed Mutagenesis Kit was used to construct five p28GANK mutants with different ankyrin repeats deleted and their influences on p53 were also investigated. Results: Five deletion mutants of p28GANK were as follows: 39-71aa, 72-104aa, 105-137aa, 138-170aa, and 171-203aa. Agarose gel electrophoresis and Western blotting analysis confirmed the correct construction of these mutants. Wild type p28GANK promoted degradation of p53, but the 5 mutants had no noticeable effects on expression of p53. Conclusion: We have successfully constructed five p28GANK ankyrin repeat-deleted mutants. It is confirmed that the regulation of p53 needs the complete ankyrin repeats of p28GANK, which paves a way for further research on ankyrin and the role of p28GANK in HCC.
