Salvia miltiorrhiza bge. f. alba up-regulates Bcl-2 and inhibits apoptosis of human umbilical vein endothelial cells
10.3724/SP.J.1008.2010.01211
- Author:
Chang-Kai YU
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Bcl-2 genes;
Endothelial cell;
Salvia miltiorrhiza bge. F. Alba;
Umbilical veins
- From:
Academic Journal of Second Military Medical University
2010;31(11):1211-1214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of Salvia miltiorrhiza bge. f. alba(SMA) on apoptosis and Bcl-2 expression in human umbilical vein endothelial cell (HUVEC) in vitro. Methods HUVECs were isolated using perfusion and enzyme digestion methods, and the obtained cells were identified by morphological observation and VIII = Ag immunoreactivity examination. The cells in the exponential phase of growth were treated with H2O2 and different concentrations of SM A (high dose, 0.10 g/ml, low dose, 0.01 g/ml). The cell apoptosis was determined by flow cytometric analysis and Bcl-2 expression was examined by immunofluorescence mehtod. Results SMA significantly decreased the H2O2-induced apoptosis of HUVECs (P<0.01 in high dose group and P<0.05 in low dose group), and significantly increased the expression of Bcl-2 in high dose group(P<0.01). Conclusion SMA can inhibit H2O2-induced apoptosis of HUVEC, which might be associated with the increase of Bcl-2 expression. Salvia miltiorrhiza bge. f. alba; umbilical veins; endothelial cell; apoptosis; bcl-2 genes.
