Detection of hepatitis C virus cross-neutralization antibodies in sera of hepatitis C patients
10.3724/SP.J.1008.2010.01184
- Author:
Jin JI
1
Author Information
1. Key Laboratory of Plant Protection Resources and Pest Management
- Publication Type:Journal Article
- Keywords:
Hepatitis C;
Hepatitis C virus;
Neutralization antibodies
- From:
Academic Journal of Second Military Medical University
2010;31(11):1184-1188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the presence of neutralization antibodies in the sera of hepatitis C patients. Methods A eukaryotic expression plasmid encoding carboxyl terminal-truncated HCV envelope protein 2 (E2) was transfected into human 293T cells. ELISA method was established to examine the anti-E2 antibodies in the sera of 32 hepatitis C patients. The full-length envelope protein expression plasmid was transfected into 293T cells and the reactivity of transfectant with antiE2 IgG positive sera was analyzed by immunofluorescence. Five strains of HCV pseudotype particle (HCVpp), including H77 (la genotype), Con-1 (lb genotype), J4 (lb genotype), J6 (2a genotype), and UNK3a (3a genotype), and two strains of cell cultured HCV (HCVcc) were used to assay the neutralization activity of 12 anti-E2 positive serum samples. Results ELISA results showed that 26 of 32 serum samples were anti-E2 IgG positive, with the positive rate being 81.3%. The 12 serum samples positive for HCV RNA were all anti-E2 IgG, and the virus load was negatively correlated with the anti-E2 antibody level. The anti-E2 positive sera could neutralize the infections of five strains of HCVpp and two strains of HCVcc to various extents, and the neutralization activity was consistent with the anti-E2 antibody level. Conclusion It is suggested that HCV infection can induce protective humoral immune response, and cross-neutralization antibodies against HCV are present in sera of hepatitis C patients, which indicates a feasibility for developing vaccines that can induce broadly-reactive neutralizing antibodies.
