Effects of insulin-like growth factor-1 on cystationine-β-synthase and hydrogen sulfide in PC12 cells
10.3724/SP.J.1008.2010.01296
- Author:
Tao MENG
1
Author Information
1. Department of Neurology
- Publication Type:Journal Article
- Keywords:
Cystationine-β-synthase;
Hydrogen sulfide;
Insulin-like growth factor-1;
Mitogen-activated protein kinases
- From:
Academic Journal of Second Military Medical University
2010;31(12):1296-1299
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of insulin-like growth factor-1 (IGF-1) on expression of cystationine-β-synthase (CBS) and hydrogen sulfide(H2S) in rat adrenal pheochromocytoma cell line (PC12) and the possible signaling pathway. Methods: After PC12 cells were treated with 20,40, or 80 ng/ml IGF-1 for 24 h, fluorecence quantitative PCR was used to examined the expression of CBS gene, Western blotting analysis was used to detect the expression of CBS,ERK/MAPK protein expression, and sensitive sulphur electrode was used to analyze the levels of H2S gas. For inhibition studies, 25 or 50 mol/L PD98059, a specific ERK/MAPK inhibitor, was used 30 min prior to 80 ng/ml IGF-1 treatment: and the above parameters were examined again using the same method. Results: IGF-1 increased the expression of CBS, pERK1/2, and the levels of H2S; and PD98059 could inhibit the above effect of IGF-1. Conclusion: IGF-1 and its ERK/MAPK signaling pathway regulate the expression of CBS and levels of H2S.
