Biliary epithelial cells inhibit proliferation of co-cultured hepatic cancer cell line HepG2
10.3724/SP.J.1008.2011.00017
- Author:
Yao HE
1
Author Information
1. Department of Gastroenterology, SUN Yat-sen University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Biliary epithelial cells;
Caspase3;
Co-culture;
HepG2 cells;
Liver neoplasms
- From:
Academic Journal of Second Military Medical University
2011;32(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the possible effect of mouse intrahepatic biliary epithelial cell line (mIBEC) on co-cultured human hepatoma cell lines HepG2. Methods: HepG2 and mIBEC cells were co-cultured in a membrane-separated Transwell system. CellTiter 96 ® AQueous One Solution Cell Proliferation Assay (Promega Corporation, Madison, WI) was used to examine HepG2 cell proliferation in the system with or without co-cultured mIBEC. Real-time PCR(RT-PCR) was used to determine Ki67 and caspase3 mRNA expression in HepG2 cells in a system with or without co-cultured mIBEC. Western blotting analysis was used to determine caspase3 protein level in HepG2 cells. Results: The proliferation of the co-cultured HepG2 cells was significantly lower than those cultured alone(P<0.01). Expression of Ki67, a cell proliferation marker, was also significantly down-regulated in mIBEC co-cultured HepG2 cells (P<0.05). The levels of caspase3 mRNA and protein were significantly up-regulated in mIBEC co-cultured HepG2 cells compared with HepG2 cells cultured alone (P < 0.05). Conclusion: mIBEC can inhibit the proliferation of co-cultured HepG2, and caspase3 activation might be one of the reasons for the inhibitory effect of mIBEC against HepG2 cells.
